Poly(1-vinylimidazole) polyplexes as novel therapeutic gene carriers for lung cancer therapy

• Gayathri Kandasamy,
• Elena N. Danilovtseva,
• Vadim V. Annenkov and
• Uma Maheswari Krishnan

Beilstein J. Nanotechnol. 2020, 11, 354–369, doi:10.3762/bjnano.11.26

• that have been connected to the proliferation and invasion of lung cancer cells. These results indicate that the PVI complexes can be an effective agent to counter lung cancer. Keywords: anti-VEGF siRNA; gene silencing; lung cancer; microarray; poly(1-vinylimidazole); small interfering RNA (siRNA

• carrier. The present work aims to synthesize poly(1-vinylimidazole) for the delivery of anti-VEGF siRNA to lung cancer cells and explore for the first time the effect of VEGF silencing on differential expression of genes and on cell viability, migration and chemosensitization. Experimental Materials PVI

• absence of free siRNA in the polyplex lanes treated with heparin shows the stability of the complex formed. (A) Intracellular uptake of the polyplex monitored by using laser scanning confocal microscopy after 4 h of treatment with Cy3-labeled anti-VEGF siRNA (emission wavelength = 568 nm) at a final siRNA